Identification of Novel Subcellular Localization and Trafficking of HIV-1 Nef Variants from Reference Strains G (F1.93.HH8793) and H (BE.93.VI997)

Identification of Novel Subcellular Localization and Trafficking of HIV-1 Nef Variants from Reference Strains G (F1.93.HH8793) and H (BE.93.VI997)

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The human immunodeficiency virus type 1 (HIV-1) accessory protein Nef, plays an essential role in disease progression and pathogenesis via hijacking the host cellular membrane-trafficking machinery.Interestingly, HIV-1 group-M subtypes display differences in the rate of disease progression.However, few reports investigated how the cellular behaviors and activities of Nef isolates from reference strains may differ between HIV-1 click here group-M subtypes.Here, we characterize how differing cellular distributions of Nef proteins across group-M subtypes may impact protein function using immunofluorescence microscopy and flow cytometric analysis.

We demonstrate that Nef variants isolated from HIV-1 group-M subtypes display differences in expression, with low expressing Nef proteins from reference strains of subtypes G (F1.93.HH8793) princess polly dresses long sleeve and H (BE.93.

VI997) also displaying decreased functionality.Additionally, we demonstrate variations in the subcellular distribution and localization of these Nef proteins.Nef from subtype G (F1.93.

HH8793) and H (BE.93.VI997) reference strains also failed to colocalize with the trans-Golgi network, and were not differentially localized to cellular markers of multivesicular bodies or lysosomes.Strikingly, our results demonstrate that HIV-1 Nef proteins from reference strains G (F1.

93.HH8793) and H (BE.93.VI997) highly colocalize with labeled mitochondrial compartments.